Shuttle vectors for cloning recombinant DNA in Escherichia coli and Streptomyces griseofuscus C581

Vectors for Cloning Recombinant DNA in Escherichia coli and Streptomyces griseofuscus C 581

The streptomycete plasmid SCP2 and its mutant SCP2* (3, 4, 15) are 31-kilobase (kb) (10) sex factors (3) with one to five copies per cell (16). The SCP2 sequence can be stably cloned in Escherichia coli (12). SCP2* is suitable as a cloning vector in streptomycetes (2). Further developments of SCP2* into useful cloning vectors are described in this report. (Portions of this work were presented a...

Optimization of Cloning Conditions for high-level Production of Recombinant Mouse Interleukin-2 in Escherichia coli

Backgrounds and objectives: Interleukin 2 (IL-2) secreted by activated CD4+ T cells  has been known as a major mediator in both  adaptive and native immune system  due to a board range of effects on  different cells in the immunity system (1-6). Methods: cDNA synthesis was performed using gene- specific primers designed by Gene Runner software after RNA extraction of mouse splenocytes. PCR pro...

Expression Cloning of Recombinant Escherichia coli lacZ Genes Encoding Cytoplasmic and Nuclear P-galactosidase Variants

Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...

Advances and challenges in recombinant protein expression in Escherichia coli

Novel cloning method for recombinant adenovirus construction in Escherichia coli.

pAd(vantage) is a rapid cloning system for generating recombinant adenoviruses. The system is based on manipulating the full-length adenovirus genome as a stable plasmid in E. coli using intron-encoded endonucleases. These intron-encoded endonucleases cut their recognition sequences, which range from 15-39 bp, with high specificity. Their unusual long homing sequence makes them rare-cutting and...